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What will be the first commercial product utilizing ClpX unfoldase and CsgG nanopore protein reading technique by end of 2025?
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Groundbreaking Single-Molecule Protein Reading Using ClpX Unfoldase and CsgG Nanopore Published in Nature
Sep 11, 2024, 04:19 PM
A groundbreaking approach for single-molecule protein reading has been published in Nature. The technique utilizes ClpX unfoldase to ratchet proteins through a CsgG nanopore, achieving single-amino-acid sensitivity. This advancement, described by researchers including Jeff Nivala and the team from ONT, represents a significant leap in protein sequencing technology. The method allows for multi-pass, single-molecule reading of long protein strands, and can detect post-translational modifications. It requires chemical conjugation of functional amino acids. This development is expected to have profound implications for biological research and understanding protein structures.
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