New CRISPR Tool HACE Enables Long-Range Targeted Mutagenesis in Mammalian Genomes Published in Science Magazine

Oct 11, 2024, 02:30 PM

A new CRISPR-based tool called Helicase-Assisted Continuous Editing (HACE) has been developed for long-range, targeted mutagenesis of over 1000 base pairs in mammalian genomes. This tool allows researchers to explore the effects of genetic variants on gene function and regulation in both coding and non-coding regions. The research, co-authored by Brad E. Bernstein from Dana-Farber Cancer Institute and published in Science Magazine, expands the functional genomics toolbox. It demonstrates that HACE can continuously generate mutations over a 10-day period and enables targeting using dCas9 without introducing DNA nicks.