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For which non-human organisms will the FindingNemo Toolkit be adapted by September 30, 2025?
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Resolution / Starting Odds
Plants • 25%
Animals • 25%
Microorganisms • 25%
Other • 25%
Scientific publications or official announcements
Story
FindingNemo Toolkit Enhances Ultra-Long DNA Sequencing in a Single Day
Aug 19, 2024, 01:15 PM
A new toolkit named FindingNemo has been developed for DNA extraction, library preparation, and purification for ultra-long nanopore sequencing. The protocol, which is a result of a collaborative effort involving researchers from institutions such as DeepSeqNotts, can be adapted to work with as few as one million human cell equivalents or 5 micrograms of ultra-high molecular weight DNA. It allows for extraction to sequencing in a single working day. The toolkit is designed to produce high occupancy ultra-long reads on nanopore platforms, offering throughput comparable to disc-based methods and potential advantages for tissues and non-human cell materials. This development also aids in understanding allele-specific expression in single-nucleus RNA-seq data.
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Rabbits • 25%
Pigs • 25%
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Environmental science • 25%
Evolutionary biology • 25%
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Plant • 25%
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Fungus (other than mushroom) • 25%
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No • 50%
Yes • 50%
No • 50%
Yes • 50%
No • 50%
Discovery of new regulatory mechanisms • 25%
Identification of new genome regions • 25%
New insights into evolutionary history • 25%
Other • 25%